Expression of cytoskeletal binding protein 4. 1N in Ewing's sarcoma and its clinical significance / 临床与实验病理学杂志

Purpose To investigate the expression of cy-toskeletal protein 4. 1N in Ewing's sarcoma and the correlation with cell proliferation as well as clinicopathological characteris-tics. Methods Immunohistochemical EnVision two-step stai- ning was used to detect the expression of 4. 1N in 52 Ewing's sarcoma cases, which 47 cases of them were performed to detect the expression of Ki-67. The relationship between the expression of 4. 1N and Ki-67, and the correlation with the pathological fea- tures including prognosis were analyzed. Results The positive expression of 4. 1N was found in 35% (18/52) of Ewing's sar-coma. Lower frequency of 4. 1N strong expression (1. 9% , 1/52) was detected in the cohort. Ki-67 labeling index in Ewing's sarcoma varied from 3% to 85% . 4. 1N expression displayed a negative correlation with Ki-67 index ( P ＜0. 05). Among the follow-up of 27 cases, Kaplan-Meier survival analysis suggested that patients with 4. 1N-negative had shorter survival period than patients with 4. 1N positive expression (P ＜0. 05). However, the expression of 4. 1N was not statistically correlated with sex, age, tumor's localization and metastasis. Conclusion 4. 1N mainly showed negative or lower expression in Ewing's sarcoma and was related to cell proliferation index Ki-67, which implied a potential function in regulating proliferation in Ewing's sarcoma. 4. 1N expression has potentially predictive value in outcome of E-wing's sarcoma.

Study on Reproductive Biology of Polygonatum cyrtonema Hua / 中国中医药信息杂志

Objective To study the reproductive biology of Chinese herbal medicine Polygonatum cyrtonema Hua. Methods The flowering habit of P. cyrtonema was observed and recorded regularly. The pollen viability at different flowering periods was detected by TTC. The stigma activity at different flowering periods was measured by the benzidine-hydrogen peroxide method. The hybridization index was estimated and pollen-ovule ratio was calculated. The breeding system of P. cyrtonema was determined by six different artificial pollination treatments. Results The flower buds of P. cyrtonema appeared at April 5. They bloomed at April 10 and fruited early in May, flowering about 30–40 days. The pollen began to have viability since the anthers were not cracked, and loss viability after flowers withered. The pollen viability at the bloom day was about 73.06%±5.19%. The stigmas began to have activity before flowers opening and lost it when the flowers began to decline. The hybridization index was 4, so the breeding system of P. cyrtonema Hua was partly self-compatible and xenogamy needing pollinator according to Dafni's standard.The pollen-ovule ratio was 1799.20±445.54,therefore the breeding system of P.cyrtonema Hua was facultative xenogamy according to Cruden's standard. The results of artificial pollination experiments showed that P. cyrtonema Hua had three breeding methods,including apomixis,selfing and outcrossing.The fruit setting rate of P. cyrtonema Hua was greatly influenced by external conditions such as strong wind and rain.Generally,P.cyrtonema Hua had a low fruit-set percentage because of obvious flower and fruit dropping. The natural fruit setting rate was only 17.46%. Conclusion The coexistence of apomixes, sexual and asexual reproduction in P. cyrtonema are the survival strategy adapting to different habitat types.

Study on Reproductive Biology of Polygonatum cyrtonema Hua / 中国中医药信息杂志

Objective To study the reproductive biology of Chinese herbal medicine Polygonatum cyrtonema Hua. Methods The flowering habit of P. cyrtonema was observed and recorded regularly. The pollen viability at different flowering periods was detected by TTC. The stigma activity at different flowering periods was measured by the benzidine-hydrogen peroxide method. The hybridization index was estimated and pollen-ovule ratio was calculated. The breeding system of P. cyrtonema was determined by six different artificial pollination treatments. Results The flower buds of P. cyrtonema appeared at April 5. They bloomed at April 10 and fruited early in May, flowering about 30–40 days. The pollen began to have viability since the anthers were not cracked, and loss viability after flowers withered. The pollen viability at the bloom day was about 73.06%±5.19%. The stigmas began to have activity before flowers opening and lost it when the flowers began to decline. The hybridization index was 4, so the breeding system of P. cyrtonema Hua was partly self-compatible and xenogamy needing pollinator according to Dafni's standard.The pollen-ovule ratio was 1799.20±445.54,therefore the breeding system of P.cyrtonema Hua was facultative xenogamy according to Cruden's standard. The results of artificial pollination experiments showed that P. cyrtonema Hua had three breeding methods,including apomixis,selfing and outcrossing.The fruit setting rate of P. cyrtonema Hua was greatly influenced by external conditions such as strong wind and rain.Generally,P.cyrtonema Hua had a low fruit-set percentage because of obvious flower and fruit dropping. The natural fruit setting rate was only 17.46%. Conclusion The coexistence of apomixes, sexual and asexual reproduction in P. cyrtonema are the survival strategy adapting to different habitat types.

Reconstruction of soft-tissue defects in hands using the free proximal posterior interrosseous artery forearm perforator flaps / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the applied anatomy of the proximal posterior interrosseous artery perforator flap (PIAP) and report the clinical results of repairing the soft tissue defects in hands.</p><p><b>METHODS</b>Between September 2007 and January 2011, 21 patients with 24 soft tissue defects in hands were repaired with the free proximal PIAP flap transplantation. The size of the flaps ranged from 2.0 cm x 1.5cm to 7cm x 5cm. The longest length of these flaps was 9 cm. 9 flaps were dissected with one additional superficial vein to anastomose with the superficial vein at the recipient sites.</p><p><b>RESULTS</b>19 flaps survived completely. Bubbles and violet color happened in 4 flaps which survived finally after partial suture removal. Flap necrosis occurred in one flap. The clinical results were satisfactory after 6-25 months of following-up, and the scars at the donor sites were not obvious.</p><p><b>CONCLUSIONS</b>The free PIAP flaps have constant, reliable blood supply, and good texture. It is a good option for repairing soft-tissue defects in the hands.</p>

Etiologic investigation of chronic granulomatous inflammation of hand by polymerase chain reaction and DNA sequencing / 中华病理学杂志

<p><b>OBJECTIVE</b>To explore the causative pathogens in littoral hand infections which exhibited chronic granulomatous inflammation, the relationship between chronic granulomatous inflammation and mycobacteria and to discuss the prospects of PCR in clinical application for diagnosis of granulomatous inflammation.</p><p><b>METHOD</b>With 16S-rDNA as the target sequence, Nest-PCR was used to detect mycobacteria directly from 37 cases of chronic granulomatous inflammations, and identified them by gene sequencing.</p><p><b>RESULTS</b>Twenty-four of 37 cases were positive for mycobacteria by Nest-PCR, in which 17 were M.marinum, 1 M.chelonae, 2 M.avium, 2 M.kansasii, and 2 M.tubercular through gene sequencing.</p><p><b>CONCLUSIONS</b>Nest-PCR combining gene sequencing proved to be a liable and sensitive method to detect Non-tubercular mycobacteria (NTM) in fresh tissue. NTM is the major factor of hand specific chronic infections other than tubercular. Pathological changes are difficult to differentiate TB from NTM and bacterial evidence was necessary.</p>

The etiological study, diagnose and therapy of hand special chronic infections in littoral area / 中华外科杂志

<p><b>OBJECTIVE</b>To explore the etiological factors of hand special chronic infections and their relationship with tuberculosis, and to give evidence for clinical diagnosis as well as treatments.</p><p><b>METHODS</b>From 2002 to 2004 pathologic inspection, acid-fast stain, bacterial cultication, mycobacterial cultivation were performed in all 29 cases of hand special chronic infections.</p><p><b>RESULTS</b>All cases showed granulomatous lesions in pathological appearance, 2 positive in acid-fast stain, 12 positive in bacteria cultivation, and 1 nocardiosis, 1 staphylococcus epidermidis, 7 M.marinum, 1 M.tuberculosis, 1 M.fortuitum, 1 M.kansasii.</p><p><b>CONCLUSIONS</b>Non-tuberculo-mycobacterium (NTM) especially M.marinum are far more important as the major factor than tuberculosis and other bacterial in hand special chronic infections. Bacteria cultivation should be routine examined for all cases.</p>